rna seq  (Novogene)

Journal: Bioactive Materials

Article Title: Small extracellular vesicle-integrated by herbal hydrogels for spatiotemporal immunomodulation and neurovascular repair following traumatic brain injury

doi: 10.1016/j.bioactmat.2026.02.056

Figure Lengend Snippet: Mechanism-specific prediction of TBI treatment by EG-gel. (A) Principal component analysis (PCA) of Sham, TBI, and EG-gel groups in transcriptomic space. (B) Volcano plots of differentially expressed genes for Sham vs TBI and TBI vs EG-gel comparisons. Red and blue points indicate significantly up and downregulated genes. (C) Venn diagrams of mRNA expression among the three groups. (D) Heat map of differentially expressed genes among the three groups. (E) KEGG pathway analysis for differentially expressed genes. (F) The top 20 of KEGG terms enrichment of RNA sequences. (G) Bar chart of the top 20 enriched GO terms of RNA sequences. RNA-seq was performed on peri-injury cortical tissue (n = 6 per group), differential expression was called using fold change ≥1.5, p-value <0.05, q-value <0.05, and group mean FPKM ≥0.5.

Article Snippet: For library construction, 1–2 μg of total RNA per sample was used. mRNA was enriched using oligo (dT) selection, and libraries were prepared using the KAPA Stranded RNA-Seq Library Prep Kit (Roche).

Techniques: Expressing, RNA Sequencing, Quantitative Proteomics

Journal: iScience

Article Title: DHODH regulates trophoblast fusion via IFITM-reduced plasma membrane fluidity: Implications for hypertensive disorders of pregnancy

doi: 10.1016/j.isci.2026.116163

Figure Lengend Snippet: Increased IFITM and decreased DHODH expression characterize the placenta in the context of HDPs (A) Volcano plot of transcriptomic changes identified by RNA-seq. Transcripts highlighted in red or blue were significantly altered ( q value < 0.05). (B) Differentially expressed genes were classified by functional enrichment analysis using the Reactome pathway database and Gene Ontology (GO) biological processes or cellular components. (C) Heatmap of mitochondria-related genes downregulated in the placenta in the context of HDPs. Genes with higher expression are shown in green, and those with lower expression are shown in red. Ctrl: premature delivery, n = 5; HDP, n = 5. (D) Expression of DHODH, OPA1, DNM1L, MFN1, TFAM, and IFITM1-3 in trophoblast BeWo cells treated with forskolin (FSK, 2.5 μM) and rotenone (Rote, 50 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗ p < 0.05, ∗∗ p < 0.01 vs. FSK alone (Tukey’s test). (E) Expression of IFITMs in BeWo cells treated with FSK (2.5 μM), orludodstat (Orlu, 1 nM), or brequinar (Bre, 25 nM) for 48 h. GAPDH was used as a reference gene. The data are presented as the mean ± SEM from three independent experiments. ∗∗ p < 0.01 vs. Ctrl; † p < 0.05, †† p < 0.01, ††† p < 0.001 vs. FSK alone (Tukey’s test).

Article Snippet: • Raw RNA-seq data derived from human placental samples and trophoblast cell lines have been deposited at the DNA DataBank of Japan (DDBJ) Sequence Read Archive as DDBJ: DRA021720 and DRA021721 and are publicly available as of the date of publication.

Techniques: Expressing, RNA Sequencing, Functional Assay

Journal: iScience

Article Title: DHODH regulates trophoblast fusion via IFITM-reduced plasma membrane fluidity: Implications for hypertensive disorders of pregnancy

doi: 10.1016/j.isci.2026.116163

Figure Lengend Snippet: DHODH regulates IFITM expression via IRF1 (A–H) BeWo cells or DHODH-KD BeWo cells were treated with FSK (2.5 μM), Orlu (1 nM), or Bre (25 nM) for 48 h. (A) Volcano plot showing transcriptomic changes identified by RNA-seq. Transcripts highlighted in red or blue were considered differentially expressed, as indicated by an expression change ≥2-fold ( p < 0.05). (B) Correlation analysis of RNA-seq data from Orlu-, Bre-treated, and DHODH-KD cells. (C) Differentially expressed genes were classified by functional enrichment analysis using the Wiki pathway database and GO biological processes or cellular components. (D) RNA-seq was used to evaluate the expression levels of genes associated with syncytialization. (E) RNA-seq was used to evaluate the expression levels of IRF family genes. (F) Immunoblotting for IRF1, total IRF3, and p -IRF3. GAPDH was used as a loading control. Representative data from three independent experiments are shown. The graph shows the total IRF3 and p -IRF3 levels normalized to GAPDH levels from three independent experiments. ∗∗ p < 0.01, ∗∗∗ p < 0.001 vs. Ctrl (Tukey’s test). Values represent the mean ± SEM. (G) ChIP assay showing IRF1 binding to upstream regulatory regions (up to 3 kbp) of the IFITM1, IFITM2, and IFITM3 loci in BeWo cells treated with FSK alone (2.5 μM) for 48 h. ∗ p < 0.05 vs. Ctrl; † p < 0.05, †† p < 0.01, ††† p < 0.001 vs. FSK alone (Tukey’s test). (H) Immunofluorescence staining of IRF1 (red). Nuclei were counterstained with DAPI (blue). Scale bars, 5 μm. The graph shows the number of staining cells from three independent experiments. Values represent the mean ± SEM. ∗∗∗ p < 0.001 vs. FSK.

Article Snippet: • Raw RNA-seq data derived from human placental samples and trophoblast cell lines have been deposited at the DNA DataBank of Japan (DDBJ) Sequence Read Archive as DDBJ: DRA021720 and DRA021721 and are publicly available as of the date of publication.

Techniques: Expressing, RNA Sequencing, Functional Assay, Western Blot, Control, Binding Assay, Immunofluorescence, Staining